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. 2020 Jan 16;14:217–231. doi: 10.2147/DDDT.S227651

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© 2020 Jia et al.

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Expression of Adeno, SRY and KGF in intestinal tissue. Female SD rats were induced by TNBS to establish UC model, and then treated with MSCs, which untreated MSCs (MSCs), MSCs carrying empty vector (MSCs-vec) and MSCs carrying KGF gene (MSCs-KGF), respectively. UC rats in the Model group were treated with only DMEM medium. SD female rats treated without any intervention were used as a control group. After 1 week of treatment, the expression of Adeno (A) and SRY (B) in intestinal tissues was detected by qRT-PCR. At the same time, the expression of KGF mRNA and protein in the intestinal tissues of each group was examined by qRT-PCR and Western blot assay (C). (D) Immunohistochemistry images from the intestinal tissues of rats for KGF. Scale bar = 50 μm. n=10. Data shown are mean±SD. ^##p<0.01vs Model group; ^$$p<0.01vs MSCs group.

Abbreviations: UC, ulcerative colitis; MSCs, mesenchymal stem cells; KGF, keratinocyte growth factor.