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. 2020 Jan 16;13:497–511. doi: 10.2147/OTT.S237410

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© 2020 Wang et al.

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EMT reversion by Snail silencing. (A) Snail expression detected by Real Time PCR and Western blot analysis. (B) mRNA expression of EMT related proteins assessed by quantitative real-time PCR and quantitative analyses of GAPDH expression. The expression of EMT related proteins detected by Western blot. (C, D) Wound-healing (100× magnification) and Transwell assays (200× magnification) were used to measure migration and invasion abilities after Snail silencing in FaduRR cells. Data are the Mean ± SD. n=3. *P<0.05, **P<0.01, ***p<0.001 vs si-NC group.

Abbreviations: EMT, epithelial mesenchymal transformation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; si-NC, negative control siRNA; si-Snail, Snail-specific siRNA.