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. 2019 Dec 9;295(3):868–882. doi: 10.1074/jbc.RA119.010340

Figure 3.

Figure 3.

PAS600–IL-1Ra reduces MSU-crystal peritoneal inflammation. A, graphical abstract of experimental MSU-induced peritonitis. 1) MSU crystals stimulate the release of inflammatory cytokines from 2) resident peritoneal macrophages, including IL-1β, IL-6, KC, and G-CSF. 3) Secreted cytokines entering the circulation stimulate neutrophil release from the bone marrow, which enters the bloodstream and reaches the peritoneal cavity. 4) The inflammatory milieu in the peritoneal cavity stimulates further cytokine and MPO release from infiltrating neutrophils. 5) Anakinra or PAS–IL-1Ra pretreatment inhibits IL-1 signaling in resident macrophages, the bone marrow, and infiltrating neutrophils, reducing the host inflammatory response. B, mean i.p. fluid MPO 6 h after MSU crystal challenge. C, mean i.p. fluid human IL-1Ra (in pg/ml or pm, see under “Experimental procedures”) in PAS600–IL-1Ra–treated mice 6 h after MSU challenge. B and C, n = 5, one point represents one mouse. Data presented as mean ± S.E.; *, p < 0.05 (two-tailed Student's t test).