Figure 4.

Comparison of 5-day PAS800–IL-1Ra and 1-day anakinra pretreatment efficacy in MSU-crystal–induced peritonitis. A, peritoneal leukocyte populations were assessed in i.p. fluid by automated cell counter (vehicle ranges: lymphocytes, 0.83–2.24 × 10⁶/ml; monocytes, 0.44–0.84 × 10⁶/ml; granulocytes, 1.08–2.02 × 10⁶/ml). Mouse IL-6 (B) (vehicle range 138–8620 pg/ml), mouse G-CSF (C) (vehicle range 111–559 pg/ml), mouse MPO (D) (vehicle range 2.42–39.80 ng/ml), and human IL-1Ra (E) in the i.p. fluid 4 h after i.p. instillation of MSU (120 mg/kg) are shown. B–D, data are expressed as percent change from the vehicle group mean of each experiment, with the vehicle group mean set at 100%. Then, cytokine levels or cell counts from each mouse were expressed as percentage of the vehicle mean. Experiments 1 (5 mice) and 2 (5 mice) were then combined; one point represents one mouse. Data presented as mean ± S.E.; ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001 (vehicle/PAS800–IL-1Ra comparison); #, p < 0.05; ##, p < 0.01; and ###, p < 0.001 (anakinra/PAS800–IL-1Ra comparison) (two-tailed Student's t test).