Figure 7.

Comparison between anakinra and PASylated IL-1Ra in A549 cells and human PBMC. A and B, A549 cells were stimulated for 24 h with recombinant human IL-1α. Experimental n = 4, vehicle IL-6 range 2.03–12.62 ng/ml (A) and vehicle IL-8 range 13.2–36.3 ng/ml (B). C, PBMC from six healthy donors, stimulated 5 days with heat-killed C. albicans. Vehicle IL-22 range 64.6–6290 pg/ml. A–C, equimolar anakinra, PAS600–IL-1Ra and PAS800–IL-1Ra treatments of 5.8–580 nm are indicated below bar graphs. These values were calculated from MS values (Fig. S2). Data are expressed as % change from the vehicle group, with the mean of the vehicle group triplicate set at 100%. One point represents one averaged triplicate (one experimental group). Data are presented as mean ± S.E.; *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001 (two-tailed Student's t test).