Figure 8.

MAPK pathway inhibition does not prevent sorbitol-induced SG formation and TDP-43 translocation. HEK293E cells were pretreated with the indicated kinase inhibitors before they were exposed to osmotic stress with 0.4 m sorbitol or no stress with control medium in the presence of the inhibitors. The fixed cells were immunostained with rabbit anti-TDP-43 (green) and mouse anti-eIF3η (red), and nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 20 μm.