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Schematic diagram of the cellular 3D ringed lamellar construct with the direction and area of Z-stack imaging indicated (A). Representative confocal microscopy images for annulus fibrosus (AF) cell orientation within the PCL:PLLA electrospun tubular construct using calcein stain (green) after 3 days (B) and 21 days (C). White arrows indicate cells' direction (Scale bars = 500 μm at x65 magnification).
