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. 2020 Jan 14;7:437. doi: 10.3389/fbioe.2019.00437

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Copyright © 2020 Shamsah, Cartmell, Richardson and Bosworth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro response of annulus fibrosus (AF) cells on PCL:PLLA electrospun bilayer scaffolds. Schematic diagrams representing the confocal microscopy image of Rhodamine fibers (red) with criss-cross direction; AF live cells (green) stained with Calcein; and merged image of both cells and fibers. Confocal microscopy images represent cell alignment at low and high magnifications within and on the bilayer scaffolds at day 1, 7, and 14 in culture [Scale bars: low magnification images (x60) = 750 μm; high magnification (inset) images (x500) = 100 μm]. White arrows indicate main direction of underlying fibers.