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. 2020 Jan 14;7:437. doi: 10.3389/fbioe.2019.00437

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Copyright © 2020 Shamsah, Cartmell, Richardson and Bosworth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro viability of bovine annulus fibrosus (AF) cells within the 3D ringed electrospun PCL:PLLA scaffold after 3 and 21 days. (A) Representative confocal images of live cells (green; calcein staining) and dead cells (red; ethidium staining) (n = 3; Magnification = x120; Scale bar = 250 μm); (B) Semi-quantitative analysis of cell counts taken from three representative Live/Dead images (C) Quantitative analysis of metabolic activity (n = 3), presented as fluorescence intensity and (D) Quantitative analysis of DNA content (n = 3), presented as fluorescence intensity. Data reported as mean ± SD with unpaired t-test followed by Welch's correction (p < 0.05; n = 3).