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. 2020 Jan 14;7:437. doi: 10.3389/fbioe.2019.00437

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Copyright © 2020 Shamsah, Cartmell, Richardson and Bosworth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) A modified (number of images stitched together) confocal microscopy image representing the top view of the PCL:PLLA electrospun 3D ringed lamellar construct (Magnification x25; Scale bar = 1 mm). The dashed square indicates the position used for higher magnification imaging (B) Representative confocal microscopy Z-stack projected images after 3 and 21 days for annulus fibrosus (AF) cells: Hoechst staining for cell nuclei (blue), Rhodamine staining for electrospun fibers and merged image (Magnification x60; Scale bar = 500 μm).