Table 1.

Prioritization of putative drug targets (elucidated via gene essentiality and homology analyses) in terms of druggability, virulence, and broad-spectrum distribution.

Putative drug target list		Prioritization of putative drug targets
Locus ID	Description	Virulence analysis	Druggability analysis	Broad spectrum analysis
$\text{KPN}\_02230$	$kdsA$	+	12	107
$\text{KPN}\_00194$	$lpxA$	+	2	107
$\text{KPN}\_00100$	$lpxC$	+	6	101
$\text{KPN}\_00236$	$gmhA$	+	1	107
$\text{KPN}\_03963$	$hldD$	+	2	58
KPN_01284	fabI	–	27	117
KPN_00478	purE	–	1	154
$\text{KPN}\_02000$	$ribC$	–	1	95
$\text{KPN}\_00020$	$ribF$	–	3	94
$\text{KPN}\_00367$	$ribH$	–	14	128
$\text{KPN}\_03599$	$murA$	–	7	200
$\text{KPN}\_04350$	$murB$	–	2	56
$\text{KPN}\_00095$	$murC$	–	3	100
$\text{KPN}\_00092$	$murD$	–	8	59
$\text{KPN}\_00089$	$murE$	–	3	110
$\text{KPN}\_00090$	$murF$	–	1	69
$\text{KPN}\_00094$	$murG$	–	1	78
$\text{KPN}\_04256$	$murI$	–	3	61
$\text{KPN}\_04135$	$glmU$	–	5	149
$\text{KPN}\_04352$	$coaA$	–	3	59
$\text{KPN}\_03974$	$coaD$	–	5	148
KPN_00141	panB	–	2	138
KPN_00140	panC	–	5	130
KPN_00139	panD	–	2	94
$\text{KPN}\_03979$	$dfp$	–	2	151
$\text{KPN}\_02812$	$dapA$	–	1	139
$\text{KPN}\_00039$	$dapB$	–	2	96
$\text{KPN}\_00179$	$dapD$	–	5	67
$\text{KPN}\_01096$	$tmk$	–	1	97
KPN_01074	pyrC	–	4	59
KPN_03983	pyrE	–	2	53
KPN_01277	pyrF	–	3	92
$\text{KPN}\_01228$	$nad{E}^{*}$	–	6	78
$\text{KPN}\_03799$	$asd$	–	3	63
$\text{KPN}\_02202$	$galU$	+	–	208
$\text{KPN}\_00192$	$lpxD$	+	–	74
$\text{KPN}\_02493$	$ugd$	+	4	30

Targets predicted by at least two prioritization approaches are given here. The genes that satisfy all three criteria are given at the top of the table. Target candidates from human body fluid simulation (HBF) are the subset of sputum-macrophage (SM) simulation-derived results and they are given in red letters. The number of the bacterial pathogen genomes containing the potential targets and the number of interacting drugs are listed in the table.

^*All HBF medium-predicted genes in the table were also predicted to be drug targets via the generic host medium simulation, apart from nadE. The nadE gene was classified as essential by HBF and SM simulations, but it was not predicted by the generic host medium simulation.