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. 2020 Jan 14;9:461. doi: 10.3389/fcimb.2019.00461

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Copyright © 2020 Nakashima, Pardo, Miola, Murata, Paduan, Longo, Brandão de Mattos, Pereira-Chioccola, Ricci and de Mattos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrophoretic profile of fragments of B1 gene from T. gondii genomic DNA extracted from peripheral blood of blood donors, amplified by Nested PCR. In (A), the first amplification is showing a fragment of 288 base pairs; in (B), the second amplification is showing a fragment of 115 base pairs. M, molecular marker; PC, Positive Control; CN, Negative Control.