Table 2.
Summary of recently identified miRNAs associated with osteogenesis.
| miRNA | Targets gene | In vitro/in vivo | Cells/vivo model | Biological effect | Reference |
|---|---|---|---|---|---|
| miR-16-2-3p | WNT5A | In vitro | hBMSCs | Inhibit osteogenic differentiation | (Duan et al., 2018) |
| miR-21-5p | SMAD7 | In vitro | MC3T3-E1 | Promote osteoblast differentiation | (Li and Jiang, 2019) |
| miR-27b | PPAR | In vitro | hBMSCs | Promote osteogenic differentiation | (Seenprachawong et al., 2018) |
| miR-29b | BCL-2 | In vitro | Mice BMSCs | Promote osteoclast differentiation | (Sul et al., 2019) |
| miR-34c | LGR4 | In vitro | Mice BMMs | Promote osteoclast differentiation | (Cong et al., 2017a) |
| miR-92b-5p | ICAM-1 | In vitro, in vivo | Mice BMSCs, mice | Promote osteogenic differentiation | (Li et al., 2019d) |
| miR-96 | SOST | In vitro | Mice osteoblast | Promote osteoblast differentiation | (Ma et al., 2019a) |
| miR−100−5p | FGF21 | In vitro, in vivo | Mice BMMs, mice | Inhibit osteoclast differentiation | (Zhou et al., 2019a) |
| miR-125a-5p | TNFRSF1B | In vitro | RAW 264.7 OPC | Promote osteoclast differentiation | (Sun et al., 2019a) |
| miR-128 | SIRT6 | In vitro | C2C12 cells | Inhibit osteoblast differentiation | (Zhao et al., 2019b) |
| miR-130a | PPAR | In vitro | hBMSCs | Promote osteogenic differentiation | (Seenprachawong et al., 2018) |
| miR-132-3p | Smad5 | In vitro | MC3T3-E1 | Inhibit osteoblast differentiation | (Liu et al., 2019a) |
| miR-135-5p | HIF1AN | In vitro | MC3T3-E1 | Promote osteoblast differentiation | (Yin et al., 2019) |
| miR-139-3p | ELK1 | In vitro | MC3T3-E1 | Inhibit osteoblast differentiation | (Wang et al., 2018c) |
| miR-140-5p | TLR4, BMP2 | In vitro, in vivo | ASCs, rats | Promote osteogenesis | (Guo et al., 2019b) |
| miR-141 | Calcr, EphA2 | In vitro, in vivo | M-BMMs, monkey | Inhibit osteoclast differentiation | (Yang et al., 2018b) |
| miR-142-5p | PTEN | In vitro | Rat BMMs | Promotes osteoclast differentiation | (Lou et al., 2019) |
| miR-144-3p | RANK | In vitro | CD14+PBMC | Inhibit osteoclast differentiation | (Wang et al., 2018a) |
| miR-145 | SEMA3A | In vitro | hJBMMSCs | Inhibit osteoblastic differentiation | (Jin et al., 2019) |
| miR-145-5p | OPG | In vitro, in vivo | RAW-264.7, mice | Promotes osteoclast differentiation | (Chen et al., 2018) |
| miR-146a | M-CSF | In vivo | Mice with OVX | Inhibit osteoblast differentiation | (Zhao et al., 2019a) |
| miR-199a-5p | Mafb | In vitro | RAW 264.7 cells | Promote osteoclast differentiation | (Guo et al., 2018) |
| miR-218-5p | COL1A1 | In vitro | Mice BMSCs | Promote osteoblastic differentiation | (Kou et al., 2019) |
| miR-218 | Mmp9 | In vitro | RAW264.7 cells | Inhibit osteoblastic differentiation | (Guo et al., 2019a) |
| miR-200c | Myd88 | In vitro | hBMSCs | Promote osteogenic differentiation | (Xia et al., 2019) |
| miR-208a-3p | ACVR1 | In vitro, in vivo | MC3T3-E1, mice | Inhibit osteoblastic differentiation | (Arfat et al., 2018) |
| miR-210 | Runx2 | In vitro | HUCB-MSC | Promote osteoblast differentiation | (Asgharzadeh et al., 2018) |
| miR-221 | ZFPM2 | In vitro | MC3T3-E1 | Promote osteoblast differentiation | (Zheng et al., 2018) |
| miR−223−5p | HDAC2 | In vitro, in vivo | MC3T3−E1, mice | Promote osteoblast differentiation | (Chen et al., 2019b) |
| miR-338-3p | IKKβ | In vitro | RAW264.7 cell | Inhibit osteoclast differentiation | (Niu et al., 2019) |
| RANKL | In vitro | Mice BMCs | Inhibit osteoclast differentiation | (Zhang et al., 2016a) | |
| miR-342-3p | ATF3 | In vitro, in vivo | MC3T3−E1, mice | Promote osteoblast differentiation | (Han et al., 2018) |
| miR-363-3p | PTEN | In vitro | CD14+PBMC | Promote osteoclast differentiation | (Li et al., 2019a) |
| miR-367 | PANX3 | In vitro, in vivo | Mice osteoblast, mice | Promote osteoblast differentiation | (Jia and Zhou, 2018) |
| miR-376c-3p | IGF1R | In vitro | hBMSCs, | Inhibit osteogenic differentiation | (Camp et al., 2018) |
| miR-377 | RANKL | In vitro, in vivo | hBMMs, mice | Inhibit osteoclast differentiation | (Li et al., 2019b) |
| miR-383 | Satb2 | In vitro | Rat BMSCs | Inhibit osteoblastic differentiation | (Tang et al., 2018) |
| miR-494 | BMPR2/RUNX2 | In vitro | C2C12 cells | Inhibit osteoblast differentiation | (Qin et al., 2019) |
| miR-451 | YWHAZ | In vitro, in vivo | hBMSCs, mice | Inhibit osteoblast differentiation | (Pan et al., 2018) |
| miR-487b-3p | Nrarp | In vitro, in vivo | Mice osteoblasts, mice | Inhibit osteoblast differentiation | (John et al., 2019) |
| miR-874 | SUFU | In vitro, in vivo | Rat osteoblasts, rat | Promote osteoblast differentiation | (Lin et al., 2018) |
| miR-877-3p | Smad7 | In vitro | MC3T3-E1 | Promote osteoblast differentiation | (He et al., 2019) |
| miR-1225 | Keap1 | In vitro, in vivo | BMMs, mice | Inhibit osteoclast differentiation | (Reziwan et al., 2019) |
| miR-let-7c | SCD-1 | In vitro | hADSCs | Inhibit osteogenic differentiation | (Zhou et al., 2019b) |
HIF1AN, hypoxia-inducible factor 1 α inhibitor; M-CSF, macrophage colony-stimulating factor; OVX, ovariectomy; SEMA3A, semaphorin 3A; h-JBMMSCs, human jaw bone marrow mesenchymal stem cells; ICAM-1, intracellular adhesion molecule‐1; SOST, sclerostin; hADSCs, human adipose derived mesenchymal stem cells; Nrarp, notch-regulated ankyrin-repeat protein; PPAR, peroxisome Proliferator-Activated Receptor γ; ATF3, activating transcription factor 3; SCD-1, stearoyl-CoA desaturase 1; ZFPM2, zinc finger protein multitype 2; MC3T3-E1,the mouse osteoblast-like cells; SUFU, suppressor of fused gene; IGF1R, insulin growth factor 1 receptor; HUCB, human umbilical cord blood; Satb2, special AT-rich-sequence-binding protein 2; ACVR1, activin A receptor type I; BMMs, bone marrow-derived macrophages; TNFRSF1B, TNF receptor superfamily member 1B gene; RAW 264.7 OPC, RAW 264.7 osteoclast precursor cell; Mmp9, matrix metalloproteinase-9; OPG, osteoprotegerin; M-BMMs, monkey bone marrow-derived macrophages; Calcr, calcitonin receptors; EphA2, ephrin type-A receptor 2 precursor; LGR4, leucine-rich repeat-containing G-protein-coupled receptor 4.