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. 2020 Jan 20;10:739. doi: 10.1038/s41598-020-57723-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of kidney-associated proteins in urine-derived renal progenitors and Albumin transport. (A) Urine-derived renal progenitors express the renal stem cell markers- SIX2, CITED1, WT1, and CK19. Renal markers (red) and cell nuclei were stained using DAPI/Hoechst (blue). (B) Flow cytometry analysis for the key renal stem cell transcription factor SIX2 and (C) Renal stem cell surface markers CD24, CD133, and CD106 of UM27, UF31, UF45 and UM51. (D) Detailed CpG methylation profiles of the SIX2 5′-regulatory region are documented as revealed by bisulfite sequencing. Filled circles represent methylated CpG dinucleotides and white circles unmethylated CpGs. Arrows indicate the transcription start site. 1.9% of CpG dinucleotides were found to be methylated. (E) Urine-derived renal progenitors (n = 4) like the human kidney biopsy-derived hREPCs also transport Albumin. Albumin was coupled to Alexa Fluor 488 (green) and cell nuclei stained with DAPI (blue). Scale bars indicate 50 µm.