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. 2020 Jan 20;10:670. doi: 10.1038/s41598-019-57368-w

Figure 2.

Figure 2

Impact of adipose macrophage deletion on pericyte coverage and vascular density in the eWAT of HFD-fed mice. (a,c) Representative images of pericytes and endothelial cells in eWAT from mice given Veh or Clod for 2 and 6 weeks. Pericytes and endothelial cells were stained with anti-CD13 (green) and anti-PECAM-1 (red) antibodies, respectively. (b,d) Percentage of the pericyte association with endothelial cells in eWAT from mice given Veh or Clod for 2 and 6 weeks. Percentages were quantified by the ratio of the associated area (yellow) to the endothelial area. (e,f) Representative images of endothelial cells stained with the anti-PECAM-1 antibody and quantified vessel area in the eWAT of mice given Veh or Clod for 6 weeks. Regarding the 2-week treatment, n = 7–9; for the 6-week treatment, n = 8–12. Data are shown as means ± S.E. *p < 0.05, significantly different from Veh.