Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 20;10:670. doi: 10.1038/s41598-019-57368-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Relevance of MAPK signaling pathways to LPS-induced Pdgfb mRNA expression coupled with glycolysis in macrophages. (a-c) Representative images blotted with anti-phospho-ERK1/2, anti-ERK, anti-phospho-JNK, anti-JNK, anti-phospho-p38, and anti-p38 antibodies, and relative signal density of phosphorylated protein normalized with that of each total protein in RAW264.7 cells pretreated with 10 mM 2-DG for 0.5 hours and stimulated with 100 ng/mL LPS for 3 hours. Mean of data among four independent experiments. n = 4. Statistical analyses were performed among the same glucose concentration. (d) Representative images blotted with anti-phospho-ERK1/2 and anti-ERK1/2 antibodies and the relative signal density of phospho-ERK2 normalized with that of ERK2 in RAW264.7 cells pretreated with HA for 0.5 hours and stimulated with 100 ng/mL LPS for 3 hours. Mean of data among three independent experiments. n = 4. (e) Relative expression levels of Pdgfb mRNA in RAW264.7 cells pretreated with U0126 for 2 hours and stimulated with 100 ng/mL LPS for 3 hours. Mean of data among three independent experiments. n = 6. (f) Representative images blotted with anti-ERK1/2 and anti-α-Tubulin antibodies, and the relative signal density of ERK2 normalized with that of α-Tubulin in siErk2-transfected RAW264.7 cells. Mean of data among three independent experiments. n = 3. (g) Relative expression levels of Pdgfb mRNA in RAW264.7 cells stimulated with 100 ng/mL LPS for 3 hours after the siErk2 transfection. Mean of data among six independent experiments. n = 6. (h) Relative expression levels of Pdgfb mRNA in BMDMs pretreated with 2-DG or HA for 0.5 hours, or U0126 for 2 hours and stimulated with 100 ng/mL LPS for 3 hours. n = 4. Data are shown as means ± S.E. *p < 0.05 and **p < 0.01, among two groups, as indicated. (i) A schematic illustration of divergent signaling pathways for gene induction from TLR4 signaling-coupled glycolytic metabolism in macrophages. Synergy promotes the induction of Pdgfb and inflammatory cytokine genes. Pdgfb mRNA expression is induced through the ERK signaling pathway and extracellular acidosis caused by lactic acid production, whereas that of inflammatory cytokine genes is induced through p65 NFκB and mTORC1. A.U., arbitrary unit; TLR4, toll-like receptor 4; mTORC1, mechanistic target of rapamycin complex 1.