Table 1.

Primer sequences used in this study.

	Primer sequence (5′-3′)	Target region	Use	Annealing temperature (oC)	Product size (bp)
SLA1-e1F1	MTAARCTCTCCRCCCASCCGGCTCTG	5′ UTR	Typing PCR	65	1844
SLA-e4R4	cGGGTCACATGTGTCyTTGGAGG	Exon 4
SLA1-e1F1	MTAARCTCTCCRCCCASCCGGCTCTG	5′ UTR	Real time PCR	65	137
SLA-e12R	AGGGAGTGGGGACCCGCCT	Exon 1–2
GAPDH-F2	CCTGGCCAAGGTCATCCA	Exon 6	Real time PCR	53	123
GAPDH-R2	CGGCCATCACGCCACAG	Exon 7
SLA-CATF	CCAATCRGCGMMACYGCTGGTTCC	5′ UTR	Cloning for primer design	65	1939
SLA-R	GATCTCCTTAGGGTAGAAGCCCAaatta CAGCACCTCA	Exon 4
LSPE2F	CSTGTCCCGGCCCGAC	Exon 2	Cloning sequencing	50
SLA-CQ2R	TTCCTGGGGATGGGGATG	Intron 2
LSPE3F	GCGGGGTCAGGGTCTC	Exon 3
SLA-i3R2	GAGGGGAGATGGTGGAG	Intron 3
SLA1-Seq. 2-F	tgctatgctgtgCGCCGARAGGAGGGT	Intron 1	Typing sequencing
SLA1-Seq. 2-R	ACCCGGAGGTCGGGGT	Intron 2
SLA1-Seq. 3-F	atgctgattatcgCCCKGGTTGGWCGCG	Intron 2
SLA1-Seq. 3-R	TCCTCCCTCTCAGGACAG	Intron 3
T7	TAATACGACTCACTATAGGG	cloning vector	Cloning sequencing
T3	ATTAACCCTCACTAAAG
SP6	ATTTAGGTGACACTATAG

Note: Nucleotides in bold indicate degenerate bases. Sequences in lower case indicate the non-template-based regions to improve PCR and sequencing efficiency.