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. 2020 Jan 20;11(1):41. doi: 10.1038/s41419-020-2232-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative micrographs and quantification of tumor spheres formed by indicated Hep-2 and Tu-212 cells. Histograms showed the mean number of spheres formed by the indicated cells. b, c Flow cytometry analyses showed proportion of side population b and CD133⁺ population d in laryngeal cancer cells. d Real-time PCR analysis of OCT4, SOX2, and NANOG expression in FOXD2-AS1-overexpressing and -silencing cells. Expression levels were normalized to GAPDH. e Western blot analysis of OCT4, SOX2, and NANOG expression in indicated cells. GAPDH was used as a loading control. *P < 0.05, **P < 0.01, and ***P < 0.001.