Table 1.
Optical properties of the studied bead populations including measurement parameters, bead diameter D, ensemble (reference) lifetimes, and FLIM lifetimes as well as lifetimes from the LT-FCM measurements.
| Code | Luminophore | Diameter D/μm | λex/nm | λdet/nm | τint/ns | ||
|---|---|---|---|---|---|---|---|
| ensemble | FLIM | LT-FCM | |||||
| DyeG | Rh6G | 5.4 ± 0.5 | 488 | 550 | 3.5 | ||
| 485 | 525LP | 3.3 ± 0.04 | |||||
| 488 | 530LP | 9.4 ± 0.9 | |||||
| QDG | CdSe/ZnS | 6.2 ± 0.6 | 408 | 508*) | 13.5 | ||
| 485 | 525LP | 8.7 ± 0.2 | |||||
| 488 | 530LP | 21 ± 3 | |||||
| DyeR | Red5 | 6.5 ± 0.5 | 488 | 669 | 1.6 | ||
| 485 | 525LP | 1.9 ± 0.05 | |||||
| 488 | 530LP | 10.1 ± 1 | |||||
| QDR | CdSe/CdS/ZnS | 6.2 ± 0.6 | 408 | 648*) | 15.7 | ||
| 485 | 525LP | 9.8 ± 0.01 | |||||
| 488 | 530LP | 24 ± 3 | |||||
The ensemble and the FLIM-based lifetimes were calculated as intensity-weighted mean lifetimes from multiexponential decay fits. FLIM-based lifetimes were obtained from individual measurements of each lifetime code.
*Measurement at the emission maximum.