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. 2020 Jan 20;10:628. doi: 10.1038/s41598-020-57423-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Light micrographs of cultured LxSd1 cells taken with differential interference (A,B,D–F) or phase contrast optics (C). (A) Coccoids of various sizes. (B) A large cell with a diameter of 25 µm or more. (C) Several daughter cells hatching from a mother cell. Phase contrast microscopy clearly shows the cell wall of the mother cell (arrowhead). (D–F) Micrographs of the same cell (possibly at the early stationary stage) taken at different focus points. The reticulated green-colored cytoplasm is clearly shown. The small round structures (asterisks) show a differential interference effect that contrasts with that of the cell body, indicating that these are vacuoles containing fluid with a density lower than that of the cytoplasm. One large high-density granule of 2–3 μm in diameter was observed in the cell that showed the same differential interference effect as the cell body (arrowhead in (F)), which appeared to be a pyrenoid. (G–I) Micrographs of the same cell (possibly at the late stationary stage) taken at different focus points. One pyrenoid-like granule was also found in this cell (arrowheads in (G,H)), and many smaller granules of high optical density were also observed (arrows in (I)). Scale bar: 10 µm.