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. 2020 Jan 20;10:702. doi: 10.1038/s41598-020-57627-1

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T lymphocyte, macrophage and dendritic cell interactions in sarcomas. Inverted grayscale images of immunostained section detail from Case 14. PDL1+ macrophages expressing HLA-DR, CD16, CD64, CD68, CD163 and TIM3 are highlighted by a dashed line and are surrounded by PD1+ T cells, both CD8 and CD4. In the center, an HLA-DR+, S100AB+ CD14- dendritic cell is highlighted by a solid line. The empty arrowhead shows a CD8+, VISTA +, TCF7+ lymphocyte. The black arrowheads point to CD4+, FOXP3+, TCF7−, CD32±, OX40 ± regulatory T cells. The empty triangle points to a CD3+, CD8+, TCF7+, CD69+, VISTA+ lymphocyte. Note that TCF7 and FOXP3 are mutually exclusive. Negative markers are not represented. Image size 60 × 56 µm.