Skip to main content
. 2020 Jan 20;3:32. doi: 10.1038/s42003-020-0757-z

Fig. 1. Rh Bri2 BRICHOS R221E forms stable monomers and unstable oligomers.

Fig. 1

a, b Homology models of wild-type Bri2 BRICHOS a based on the proSP-C structure27,34 and Bri2 BRICHOS R221E b rendered with red for negative surface electrostatic potential (−10 kcal mol−1), light pink for near neutral, and blue for positive potential (10 kcal mol−1). The arrows point to Arg221 for wild-type Bri2 BRICHOS and Glu221 for Bri2 BRICHOS R221E. c SEC of wild type (wt) NT*-Bri2 BRICHOS and NT*-Bri2 BRICHOS R221E. Oli, oligomers; dim, dimers; mon, monomers. d SEC of isolated monomers of rh Bri2 BRICHOS R221E and rh wt Bri2 BRICHOS at low concentration (~7.5 µM, dashed curves) and high concentrations (54 µM for R221E and 30 µM for wt, solid curves). The inset shows native PAGE of rh Bri2 BRICHOS R221E monomers before (−) and after (+) overnight incubation at 37 °C in 20 mM NaPi pH 8.0. e Rh Bri2 BRICHOS R221E oligomers analysed by native PAGE before (−) and after (+) overnight incubation at 37 °C in 20 mM NaPi pH 8.0. Hex, hexamers; tet, tetramers; dim, dimers; mon, monomers.