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. 2020 Jan 20;3:32. doi: 10.1038/s42003-020-0757-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Individual fits (solid lines) of normalized and averaged aggregation traces (crosses) of 3 µM Aβ42 in the presence of 0 (black), 10 (purple), 30 (cyan), 50 (yellow), 70 (green), and 100% (blue) rh Bri2 BRICHOS R221E monomer a and dimer b with the combined rate constants $\sqrt{k_{n} k_{+}}$ and $\sqrt{k_{+} k_{2}}$ as free fitting parameters. The dependencies of the relative combined rate constants obtained from the fits (insets) reveal a strong effect of both rh Bri2 BRICHOS R221E species on secondary nucleation (k₊k₂) but not on primary (k_nk₊) pathways. c Values for r_max and τ_1/2 (inset) extracted from the fitting of Aβ42 aggregation traces in the presence of different concentrations of rh Bri2 BRICHOS R221E species as shown in a and b. d–f Seeded aggregation traces of 3 µM Aβ42 with 0.6 µM preformed Aβ42 fibrils in the presence of 0 (black), 10 (purple), 30 (cyan), 50 (yellow), 70 (green), and 100% (blue) rh Bri2 BRICHOS R221E monomer d and dimer e. f Elongation rates (k₊) determined from highly seeded aggregation kinetics in d and e. g–i 5 µM Aβ42 was incubated with and without 50% molar ratio of rh Bri2 BRICHOS R221E monomers g or dimers h overnight at 37 °C. The samples were treated with goat anti-Bri2 BRICHOS antibody and a gold-labeled secondary antibody, and characterized by TEM. The scale bars are 100 nm. The arrows indicate the fibril ends. i Number of gold particles located within 30 nm of eight Aβ42 fibril ends after cofibrillation with rh Bri2 BRICHOS R221E monomers and dimers, respectively. The data are reported as means ± standard deviation. ***p < 0.001.