Figure 2.

Bacterial microbiota are localized to the larval mouth and gut lumen. Bacterial colonization was visualized in 4-arm larvae using fluorescent 16S in situ hybridization. (A) 4 dpf laboratory-raised larvae grown in FSW + 20% 40 μm adult-exposed tank water (top, AEW) or 0.2 μm-filtered FSW + P/S (bottom, P/S). (B) 9 dpf larva raised in natural seawater. All scale bars = 50 μm. hg, hindgut; mg, midgut; mo, mouth. In the literature, the midgut and hindgut are also referred to as the stomach and intestine. Images are representative of >5 independent experiments.