Table 2.
Putatively-identified root metabolites exhibiting robust changes in response to auxin.
| Metabolite | EMRT | Method | Average log two-fold change after transfer to hormone-containing medium | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 0.5 h | 1 h | 2 h | 4 h | 8 h | 12 h | 24 h | |||
| Indole-containing metabolites including glucosinolates | |||||||||
| IAA hexose* | 306.41_336.1079 | RP-Neg | −5.2 | 1.9 | −13.9 | −1.7 | 1.1 | 2.1 | −1.8 |
| oxIAA hexose | 273.02_352.1028 | RP-Neg | 1.7 | 1.9 | 3.4 | 5.1 | 6.0 | 8.3 | 5.2 |
| 6-hydroxyindole-3-carboxylate dihexose | 172.03_500.1401 | RP-Neg | −2.9 | 2.6 | −2.9 | −1.8 | 1.6 | 2.0 | −1.2 |
| 6-hydroxyindole-3-carboxylate hexose | 190.42_338.0873 | RP-Neg | −3.1 | 1.4 | −3.2 | −1.7 | 1.2 | 1.9 | −1.6 |
| 4-methoxy-3-indolylmethyl glucosinolate | 296.17_477.0638 | RP-Neg | −1.7 | 1.1 | −4.2 | −1.8 | 1.7 | 4.1 | −1.2 |
| Phenylpropanoids | |||||||||
| quercetin hexose hexose* | 258.76_625.1406 | RP-Neg | 1.4 | 6.1 | 2.2 | 1.3 | 2.0 | −1.1 | −1.1 |
| quercetin hexose hexose deoxyhexose | 292.71_771.1982 | RP-Neg | 1.5 | 3.7 | 1.7 | 1.7 | 1.6 | −1.1 | 1.2 |
| quercetin hexose deoxyhexose deoxyhexose | 306.01_755.2032 | RP-Neg | 1.6 | 7.6 | 2.2 | 1.1 | 2.1 | 1.2 | 1.2 |
| quercetin hexose deoxyhexose (rutin) | 307.99_609.1459 | RP-Neg | 1.6 | 3.5 | 1.8 | 1.3 | 1.5 | −1.2 | 1.1 |
| quercetin deoxyhexose pentose | 314.09_579.135_ | RP-Neg | 1.6 | 4.0 | 2.1 | 1.5 | 1.8 | 1.2 | 1.2 |
| quercetin hexose | 352.44_463.0873 | RP-Neg | 1.2 | 8.1 | 4.4 | 1.9 | 2.3 | −1.5 | 1.3 |
| isorhamnetin hexose deoxyhexose | 335.2_623.1614 | RP-Neg | 1.4 | 3.5 | 2.0 | 1.6 | 1.7 | −1.1 | 1.3 |
| isorhamnetin hexose | 386.17_477.1031 | RP-Neg | 1.0 | 10.6 | 4.2 | 1.7 | 3.6 | −1.3 | 1.2 |
| kaempferol hexose deoxyhexose | 329.4_593.151 | RP-Neg | 1.3 | 2.7 | 2.0 | 1.4 | 1.4 | −1.0 | 1.3 |
| kaempferol hexose deoxyhexose deoxyhexose* | 327.01_739.2083 | RP-Neg | 1.0 | 3.8 | 3.2 | 1.1 | 2.0 | 1.1 | 1.7 |
| kaempferol deoxyhexose deoxyhexose* | 354.4_577.1557 | RP-Neg | 1.3 | 2.3 | 2.4 | 1.2 | 3.0 | −1.1 | 1.6 |
| kaempferol hexose | 379.71_447.0924 | RP-Neg | 1.3 | 4.6 | 5.7 | 2.1 | 3.6 | −1.0 | 1.4 |
| coumaric acid hexose (isomer a) | 265.14_325.092 | RP-Neg | −1.0 | 5.1 | 2.9 | −1.1 | 3.9 | −1.4 | 1.3 |
| coumaric acid hexose (isomer b) | 278.71_325.092 | RP-Neg | −1.2 | 4.0 | 1.4 | −1.2 | 4.4 | −1.2 | −1.4 |
| caffeic acid hexose | 232.68_341.0869 | RP-Neg | 1.0 | 6.5 | 2.2 | −1.4 | 4.1 | −1.4 | 1.1 |
| coumaroyl aspartate | 271.05_278.0659 | RP-Neg | 1.1 | 2.4 | 1.5 | 6.2 | 11.6 | 28.9 | 8.9 |
| Fatty acids | |||||||||
| phosphatidylcholine 34:2 | 170.7_796.523 | HILIC-Pos | 3.4 | 3.6 | −1.1 | 1.3 | −1.7 | −1.8 | −2.6 |
| phosphatidylcholine 34:3 | 171.23_756.554 | HILIC-Pos | 4.3 | 3.6 | −1.1 | 1.3 | −1.8 | −2.2 | −2.6 |
| phosphatidylcholine 36:5 | 171.16_780.554 | HILIC-Pos | 4.8 | 3.3 | −1.1 | 1.5 | −1.7 | −1.8 | −2.4 |
| phosphatidylcholine 36:4 | 170.85_782.568 | HILIC-Pos | 4.5 | 3.9 | −1.2 | 1.4 | −1.8 | −1.8 | −2.9 |
| trihydroxyhexadecanoic acid hexose* | 481.27_465.2694 | RP-Neg | 1.9 | 8.1 | −1.4 | 1.4 | −1.4 | 1.7 | 1.2 |
Included are all metabolites with features that differed ≥ 2 fold relative to the time-matched control in all three biological replicates at one or more sampling times; values that meet these criteria are shown in bold and are underlined. Values are the average fold change for the three replicates, with positive numbers indicating elevated levels and negative numbers indicating reduced levels relative to untreated controls. Putative identifications are based on fragmentation patterns obtained by MS/MS and MSE, with further details available in Table S1. Asterisks identify the five metabolites common to the auxin and ethylene datasets. RP = reversed phase; HILIC = hydrophilic interaction chromatography.