Table 1.
Properties of potato peptides predicted by bioinformatics to have emulsifying activity.
| Peptide | Sequence | Score* | AA Separation** | Accession Number(s) |
Number of residues |
α-helical content, %*** | β-strand content, %*** | Purity****, % | Mw, g/mol | pI***** | Net charge***** (pH 7) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| α-10 | KKPVSKDSPETYEEALKRFAKLLSDRKKL | 2.176 | — | P15477 P11768 Q42502 Q3YJT3 | 29 | 26 | 7 | 92.7 | 3405.9 | 10.38 | 4 |
| α-11 | EALKRFAKLLSD | 3.522 | — | P15478 P15477 Q3YJT5 P11768 Q2MY60 Q2MY43 Q42502 Q3YJT3 | 12 | — | — | 97.0 | 1390.6 | 9.92 | 1 |
| α-12 | DSPETYEEALKRFAKLLSD | 3.037 | — | P15477 P11768 Q42502 Q3YJT3 | 19 | 74 | 5 | 94.9 | 2212.4 | 4.16 | −2 |
| α-13 | NRPFAAAKDIVPFYFEHGPHIFN | 2.155 | — | P15477 P11768 Q42502 Q3YJT3 | 23 | — | — | 95.1 | 2688.0 | 7.53 | 0.2 |
| α-14 | AKDIVPFYFEHGPHIFN | 2.255 | — | P15477 P11768 Q42502 Q3YJT3 | 17 | — | — | 89.5 | 2031.3 | 6.05 | -0.8 |
| α-15 | IPATILEFLEGQLQEVDNN | 2.000 | — | P15477 P11768 Q42502 Q3YJT3 | 19 | — | — | 86.3 | 2143.4 | 0.61 | −4 |
| α-16 | ILEFLEGQLQEVDN | 2.978 | — | P15478 P15477 P11768 Q42502 Q3YJS9 Q3YJT3 | 14 | — | — | 83.7 | 1646.8 | 0.61 | −4 |
| α-17 | KYDGKYLMQVLQE | 2.029 | — | P15478 Q3YJT5 Q2MY60 Q2MY43 Q3YJS9 | 13 | — | — | 89.8 | 1614.9 | 6.67 | 0 |
| α-18 | KYLMQVLQEKLGE | 2.022 | — | P15478 Q3YJT5 Q2MY60 Q3YJS9 | 13 | — | — | 96.8 | 1578.9 | 6.77 | 0 |
| α-19 | KYLMQVLQEKL | 2.058 | — | P15478 Q3YJT5 Q2MY60 Q3YJS9 | 11 | — | — | 74.5 | 1392.7 | 9.49 | 1 |
| β-20 | ELDSRLSYRIISTFWGALGGDVYLGKSPN | 2.630 | — | Q3S474 A0A097H149 Q66LL8 Q66LL2 | 29 | — | — | 75.0 | 3215.6 | 6.87 | 0 |
| β-21 | ELDSRLSYRIISTFWGALGGDVYL | 3.131 | — | Q3S474 A0A097H149 Q66LL8 Q66LL2 | 24 | — | — | 70.0 | 2732.0 | 4.17 | −1 |
| β-22 | CPFSSDDQFCLKVGV | 2.501 | — | Q3S474 A0A097H149 L0AQL9 Q2XPX8 Q2XPY0 | 15 | 13 | 33 | 78.9 | 1644.9 | 3.71 | −1.1 |
| β-23 | FIPLSTNIFEDQLLNIQFNIPT | 2.391 | — | Q3S488 Q3S482 Q3S479 H9B8I8 | 22 | — | — | 89.9 | 2577.9 | 0.69 | −2 |
| β-24 | LNIQFNI | 4.066 | — | Q8RXA3 Q3S489 Q3S488 Q3S481 Q38M77 Q3S482 Q3S479 H9B8J1 H9B8I9 H9B8I8 H9B8J0 | 7 | — | — | 95.3 | 861 | 3.66 | 0 |
| β-26 | GKELDPRLSYRI | 2.218 | — | Q3S477 Q2XPX8 Q2XPY0 Q8LJQ0 | 12 | — | — | 86.9 | 1446.7 | 9.73 | 1 |
| β-27 | LNIQFNIPTPKLC | 2.213 | — | Q3S488 Q8RXA3 Q3S482 Q3S479 H9B8J1 | 13 | 23 | 31 | 70.6 | 1500.8 | 8.84 | 0.9 |
| β-28 | VHQNGKRRLALVKDNPLDVSFK | 2.056 | — | Q3S474 A0A097H149 Q2XPX8 Q2XPY0 | 22 | — | — | 94.2 | 2534.9 | 10.92 | 3.1 |
| β-29 | IGSSSHFGPHIFEGELLNIQFDIS | 2.027 | — | Q3S477 Q2XPX8 Q2XPY0 Q8LJQ0 | 24 | — | — | 84.7 | 2644.9 | 4.3 | −2.8 |
| β-30 | DDNFCAKVGVVIQ | 2.016 | — | Q3S489 Q3S488 Q3S481 Q38M77 Q3S479 H9B8I9 | 13 | — | — | 80.3 | 1407.6 | 3.71 | −1.1 |
| β-31 | LGGDVYLGKSPNSDAPCP | 2.011 | — | Q8RXA3 Q3S488 Q3S481 Q38M77 Q3S482 Q3S479 H9B8I9 H9B8I8 H9B8J0 | 18 | — | — | 91.8 | 1789.9 | 3.71 | −1.1 |
| γ-1 | GIKGIIPAIILEFLEGQLQEVDNNKDAR | 4.146 | 14 | P15477 P11768 | 28 | 75 | 0 | 99.4 | 3094.5 | 4.2 | −2 |
| γ-34 | CRDDNFCAKVGVVI | 3.387 | 5 | Q3S488 Q3S481 Q38M77 Q3S489 Q3S479 H9B8I9 | 14 | — | — | 89.0 | 1538.8 | 5.9 | −0.1 |
| γ-35 | RDDNFCAKVGVVI | 3.933 | 4 | Q3S488 Q3S481 Q38M77 Q3S489 Q3S479 H9B8I9 | 13 | — | — | 87.8 | 1435.7 | 6.05 | −0.1 |
| γ-36 | FDVIGGTSTGGLLTAMITTPNENNRP | 3.190 | 17 | P15477 Q3YJT5 P11768 Q2MY60 Q42502 Q3YJT3 | 26 | 31 | 23 | 78.6 | 2676.9 | 3.93 | −1 |
| γ-37 | LLTAMITTPNENNRP | 4.037 | 6 | P15477 Q3YJT5 P11768 Q2MY60 Q42502 Q3YJS9 Q3YJT3 | 15 | — | — | 77.6 | 1684.9 | 6.86 | 0 |
| γ−38 | FCLKVGVVHQNGKRRLALVKDNP | 3.168 | 8 | Q3S474 Q2XPX8 A0A097H149 Q2XPY0 | 23 | 0 | 65 | 89.7 | 2592.1 | 10.91 | 4 |
| γ-39 | HQNGKRRLALV | 4.790 | 7 | Q3S474 Q3S477 Q2XPX8 A0A097H149 Q2XPY0 Q66LL8 Q66LL2 Q8LJQ0 | 11 | — | — | 98.3 | 1291.5 | 12.13 | 3.1 |
| γ-40 | SSDDQFCLKVGVV | 3.137 | 5 | Q3S474 Q2XPX8 A0A097H149 Q2XPY0 L0AQL9 | 13 | 0 | 46 | 80.6 | 1396.6 | 3.71 | −1.1 |
| γ-41 | KDNPETYEEALKRFAKLLS | 3.066 | 13 | P15478 Q3YJT5 Q2MY60 Q2MY43 | 19 | — | — | 70.5 | 2252.5 | 6.88 | 0 |
| γ-42 | DTNGKELNPNSSYRIISIGRGALGGDVYL | 3.055 | 14 | Q3S481 Q38M77 Q3S489 H9B8I8 H9B8J0 | 29 | — | — | 79.0 | 3080.4 | 6.89 | 0 |
| γ-43 | NPNSSYRIISI | 3.401 | 7 | Q3S481 Q38M77 Q3S489 H9B8I8 H9B8J0 | 11 | — | — | 98.6 | 1263.4 | 9.41 | 1 |
| γ-44 | DNFCAKVGVVIQNGKRR | 3.044 | 11 | Q3S488 Q3S481 Q3S489 Q3S479 H9B8I9 | 17 | — | — | 72.3 | 1904.2 | 10.68 | 2.9 |
| γ-45 | VGVVIQNGKRR | 4.779 | 5 | Q8RXA3 Q3S488 Q3S481 Q3S489 Q3S482 Q3S479 H9B8J1 H9B8I9 H9B8J0 | 11 | — | — | 94.6 | 1225.5 | 12.13 | 3 |
| γ-46 | FAKLLSDRKKLRANK | 3.043 | 5 | P15478 P15477 P11768 Q2MY60 Q2MY43 Q42502 Q3YJS9 Q3YJT3 | 15 | — | — | 95.2 | 1788.2 | 11.57 | 5 |
| γ-47 | TPNENNRPFAAAKDIV | 3.024 | 8 | P15477 Q3YJT5 P11768 Q42502 Q3YJT3 | 16 | — | — | 97.3 | 1756.9 | 6.62 | 0 |
| γ-48 | GIIPATILEFLEGQLQEVDNN | 3.003 | 11 | P15478 Q42502 Q3YJS9 Q3YJT3 | 21 | — | — | 92.2 | 2313.6 | 0.61 | −4 |
| γ-49 | FCLKVGVIHQNGKRRLALVK | 2.478 | 8 | Q3S477 Q8LJQ0 | 20 | 0 | 75 | 99.0 | 2279.8 | 11.49 | 5 |
*Predicted amphiphilic score.
**AA used by the algorithm to define the divide between the hydrophobic and hydrophilic part of the peptide.
***Fraction of residues located in a given secondary structure conformation (within the native protein) as implied by homology modelling. This was carried out only for the 9 selected peptides.
****Purity of the synthetic peptides used as received from pepMic Co., Ltd (Jiangsu, China).
*****pI and net charge were calculated by using peptide property calculator from INNOVAGEN (Innovagen AB, Lund, Sweden).