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. 2020 Jan 14;10:3065. doi: 10.3389/fmicb.2019.03065

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Copyright © 2020 Xie, Bai, Yang, Jiang, Zhou, Zhao, Li, Niu, Zhang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tolerance to hyperosmotic stress and mycelial morphology: WT versus ΔAoime2 mutants. (A) Comparison of colonial morphology under high-osmolarity stress between WT and ΔAoime2 mutants. (B) RGI values of WT and ΔAoime2 mutants grown on TG plates supplemented with 0.2 M NaCl or 0.5 M sorbitol. Error bars: SD from three replicates, asterisk: significant difference between mutant and WT strain (Tukey’s HSD, p < 0.05). (C) Mycelia of WT and ΔAoime2 mutant strains were examined using scanning electron microscopy (SEM). Pink arrows: deformed mycelia in ΔAoime2 mutants. Bar = 10 μm. (D) Traps produced by WT and ΔAoime2 mutant strains were examined using transmission electron microscopy. Pink arrows: ED bodies in trap cells of WT and ΔAoime2 mutant strains; blue arrows: separation of cell wall and plasma membrane in ΔAoime2 mutants.