FIGURE 6.

Comparison of extracellular proteolytic activity and transcriptional levels (RTLs) of serine-protease genes between WT and mutant strains. (A) Comparison of extracellular proteolytic activity of WT and ΔAoime2 mutants on casein plates. (B) Hyphal biomass of WT and ΔAoime2 mutants cultured in PL-4 medium for 7 days. (C) Proteolytic activity of WT and ΔAoime2 mutants quantified from 7-day-old PL-4 cultures. PMSF: serine-protease inhibitor, used at 5 mM. (D) RTLs of protease-encoding genes between WT and ΔAoime2 mutants at different time points. CK (RTL = 1) was used as the standard for statistical analysis of the RTL of each gene in the deletion mutants to that in the WT strain under a given condition. Error bars: SD from three replicates, asterisk: significant difference between mutant and WT (Tukey’s HSD, p < 0.05).