TABLE 1.
Target microbe | Target | Size (bp) | Primer | Sequence (5′-3′) | References | |
PCR for confirmation of | C. jejuni | hipO | 323 | F | ACTTCTTTATTGCTTGCTGC | Wang et al., 2002 |
C. jejuni colonies | R | GCCACAACAAGTAAAGAAGC | ||||
Campylobacter spp. | 23S rRNA | 650 | F | TATACCGGTAAGGAGTGCTGGAG | Wang et al., 2002 | |
R | ATCAATTAACCTTCGAGCACCG | |||||
PCR for identification of | C. jejuni | flhB | 549 | F | TGGCAGGCGAAGATCAAGAA | Koolman et al., 2015 |
virulence and survival-related | R | GCCAAGTAAGCTGTGCAACC | ||||
genes of C. jejuni isolates | virB11 | 329 | F | TCAGGTGGAACAGGAAGTGG | Koolman et al., 2015 | |
R | GCTTTGATCGCGTCTTCTGG | |||||
hcp | 463 | F | CAAGCGGTGCATCTACTGAA | Harrison et al., 2014 | ||
R | TAAGCTTTGCCCTCTCTCCA | |||||
cadF | 400 | F | TTGAAGGTAATTTAGATATG | Konkel et al., 1999 | ||
R | CTAATACCTAAAGTTGAAAC | |||||
pldA | 913 | F | AAGCTTATGCGTTTTT | Datta et al., 2003 | ||
R | TATAAGGCTTTCTCCA | |||||
iamA | 518 | F | GCACAAAATATATCATTACAA | Müller et al., 2006 | ||
R | TTCACGACTACTATGAGG | |||||
cdtB | 376 | F | GCTCCTACATCAACGCGAGA | Koolman et al., 2015 | ||
R | ACTACTCCGCCTTTTACCGC | |||||
crsA | 878 | F | CACAGTCAGTGAAGGTGCTT | González-Hein et al., 2013 | ||
R | ACTCGCACAATCGCTACTTC | |||||
perR | 153 | F | CCCTTCAATCTCTTTAGCGACG | An et al., 2018 | ||
R | ATACCACCACATTTGGCGCA | |||||
htrA | 130 | F | CCATTGCGATATACCCAAACTT | Bui et al., 2012 | ||
R | CTGGTTTCCAAGAGGGTGAT | |||||
PCR for confirmation of | C. jejuni | tetO | 559 | F | GGCGTTTTGTTTATGTGCG | Gibreel et al., 2004 |
antibiotic resistance of | R | ATGGACAACCCGACAGAAGC | ||||
C. jejuni isolates | gyrA | F | TTTTTAGCAAAGATTCTGAT | Zirnstein et al., 1999 | ||
265 | R1 | CAAAGCATCATAAACTGCAA | ||||
368 | R2 | CAGTATAACGCATCGCAG |