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. 2020 Jan 14;10:3066. doi: 10.3389/fmicb.2019.03066

TABLE 1.

Primes used for polymerase chain reaction (PCR) in this study.

Target microbe Target Size (bp) Primer Sequence (5′-3′) References
PCR for confirmation of C. jejuni hipO 323 F ACTTCTTTATTGCTTGCTGC Wang et al., 2002
C. jejuni colonies R GCCACAACAAGTAAAGAAGC
Campylobacter spp. 23S rRNA 650 F TATACCGGTAAGGAGTGCTGGAG Wang et al., 2002
R ATCAATTAACCTTCGAGCACCG
PCR for identification of C. jejuni flhB 549 F TGGCAGGCGAAGATCAAGAA Koolman et al., 2015
virulence and survival-related R GCCAAGTAAGCTGTGCAACC
genes of C. jejuni isolates virB11 329 F TCAGGTGGAACAGGAAGTGG Koolman et al., 2015
R GCTTTGATCGCGTCTTCTGG
hcp 463 F CAAGCGGTGCATCTACTGAA Harrison et al., 2014
R TAAGCTTTGCCCTCTCTCCA
cadF 400 F TTGAAGGTAATTTAGATATG Konkel et al., 1999
R CTAATACCTAAAGTTGAAAC
pldA 913 F AAGCTTATGCGTTTTT Datta et al., 2003
R TATAAGGCTTTCTCCA
iamA 518 F GCACAAAATATATCATTACAA Müller et al., 2006
R TTCACGACTACTATGAGG
cdtB 376 F GCTCCTACATCAACGCGAGA Koolman et al., 2015
R ACTACTCCGCCTTTTACCGC
crsA 878 F CACAGTCAGTGAAGGTGCTT González-Hein et al., 2013
R ACTCGCACAATCGCTACTTC
perR 153 F CCCTTCAATCTCTTTAGCGACG An et al., 2018
R ATACCACCACATTTGGCGCA
htrA 130 F CCATTGCGATATACCCAAACTT Bui et al., 2012
R CTGGTTTCCAAGAGGGTGAT
PCR for confirmation of C. jejuni tetO 559 F GGCGTTTTGTTTATGTGCG Gibreel et al., 2004
antibiotic resistance of R ATGGACAACCCGACAGAAGC
C. jejuni isolates gyrA F TTTTTAGCAAAGATTCTGAT Zirnstein et al., 1999
265 R1 CAAAGCATCATAAACTGCAA
368 R2 CAGTATAACGCATCGCAG