Table 1.
Optimized SRM parameters applied for the analysis using the QTRAP 6500 mass spectrometer
| Mode | Analyte | Q1 (m/z) | Q3 (m/z) | Dwell time (ms) | Declustering potential (V) | Collision energy potential (V) | |
|---|---|---|---|---|---|---|---|
| Negative | OTB-NAC | Quantifier | 528.9 | 400.1 | 30 | − 30 | − 25 |
| Qualifier 1 | 528.9 | 312.0 | 30 | − 30 | − 40 | ||
| Qualifier 2 | 528.9 | 208.1 | 30 | − 30 | − 40 | ||
| d5-OTB-NAC | Quantifier | 533.9 | 405.1 | 30 | − 30 | − 25 | |
| Qualifier | 533.9 | 317.0 | 30 | − 30 | − 40 | ||
| Positive | OTA/ 2′R-OTA | Quantifier | 404.1 | 239.0 | 30 | 70 | 31 |
| Qualifier 1 | 404.1 | 221.0 | 30 | 70 | 47 | ||
| Qualifier 2 | 404.1 | 102.0 | 30 | 70 | 88 | ||
| d5–OTA/ d5–2′R–d5–OTA/ d5–2'R-OTA | Quantifier | 409.1 | 239.0 | 30 | 35 | 31 | |
| Qualifier | 409.1 | 102.0 | 30 | 35 | 88 | ||
| OTB-GSH | Quantifier | 675.2 | 277.9 | 30 | 70 | 60 | |
| Qualifier 1 | 675.2 | 296.1 | 30 | 70 | 45 | ||
| Qualifier 2 | 675.2 | 600.1 | 30 | 70 | 35 | ||
| d5-OTB-GSH | Quantifier | 680.2 | 296.1 | 30 | 70 | 45 | |
| Qualifier | 680.2 | 277.9 | 30 | 70 | 60 |