Figure 4.
Aspirin suppressed cancer stemness of lung and breast cancers. (A,B) The indicated cells were treated with 1 mM aspirin for 72 h. CD44 expression was analyzed by immunofluorescence staining (A). Nuclei were observed with DAPI staining. Scale bar, 50 μm. (B) The expressions of CD44 and ALDH1A1 were examined by Western blot. (C) Colony formation assay of indicated cells treated with indicated concentrations of aspirin. (D,E) Limiting dilution transplantation assay. HCC827 cells were treated with 2 mM aspirin for 12 h prior to transplantation. The indicated numbers of viable HCC827 cells were transplanted subcutaneously into nude mice. The incidence and the photograph of tumors (D). The growth curve and weight of tumors from 1 × 106 HCC827 cells (E). The data are presented as the means ± SEM. Student's t-test was used. *P < 0.05.