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. 2020 Jan 14;10:3004. doi: 10.3389/fmicb.2019.03004

FIGURE 4.

FIGURE 4

vgrGa and vgrGd contributes to antibacterial activity of 1D1609. Each of various A. tumefaciens 1D1609 strains (indicated in the x axis) was mixed with E. coli DH10B cells expressing pRL662 to confer gentamicin resistance at 30:1 ratio in LB (A,B) or AK (C,D) media for 18 h. The survival of E. coli cells was quantified as cfu as shown on the y axis. (A) Antibacterial activity of single vgrG deletion mutants. Data are mean ± SEM of three biological replicates, similar results were obtained from three independent experiments. Two-tailed Student’s t-test was used for statistical analyses. ∗p < 0.01. (B) Antibacterial activity in multiple vgrG deletion mutants. (C) Antibacterial activity using triple vgrG mutants. (D) Single vgrG expressed in trans using pRLBla in quadruple vgrG mutant (Δ4). Data are mean ± SEM of three biological replicates (B) or four biological replicates from two independent experiments (C,D). Different letters above the bar indicate statistically different groups of strains (P < 0.05) determined by Tukey’s HSD test, based on cfu of surviving E. coli cells.