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. 2020 Jan 14;10:1278. doi: 10.3389/fgene.2019.01278

Figure 3.

Figure 3

Anti-miR-221 and anti-miR-222 have synergetic effects on promoting sensitivity to cisplatin in MDA-MB-231 cells. (A) miR-221 and miR-222 share same seed sequence. (B, C) Anti-miR-221 (B) or anti-miR-222 (C) only did not show significant effect on sensitivity to cisplatin in MDA-MB-231 cells. (D) Combination of anti-miR-221 with anti-miR-222 significantly promoted the efficiency of cisplatin in suppressing the growth of MDA-MB-231 cell. (E) 50 μM of cisplatin inhibited cell growth and induced cell apoptosis in MDA-MB-231 cell. (F) Quantitative analysis of apoptotic cells in E. (G) Flow cytometry analysis after annexin V staining in MDA-MB-231 cells further demonstrated the synergetic effects of anti-miR-221 and anti-miR-222 to increase cell sensitivity to cisplatin. (H) Quantitative analysis of apoptotic cells in G. (I) Quantitative analysis of survived living cells in G. Data are mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ns, non significant.