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. 2020 Jan 14;10:1278. doi: 10.3389/fgene.2019.01278

Figure 5.

Figure 5

SOCS1-STAT3-Bcl-2 pathway and p53-Pten signaling involved in the miR-221/222 regulation of cisplatin sensitivity in TNBC. (A) QRT-PCR analysis of mRNA levels of SOCS1 and p27, two target gene of miR-221/222 in TNBC, and their downstream genes including STAT3 and Bcl-2 in MDA-MB-231 cells after treatment with anti-miR-221/222 or control. (B) Western blot showing upregulation of p27 and SOCS1 after knockdown of miR-221/222 in MDA-MB-231 cells. β-actin served as loading control. (C) Western blot showing suppression of STAT3, Bcl-2 and c-Myc after knockdown of miR-221/222 in MDA-MB-231 cells. β-actin served as loading control. (D) Western blot showing upregulation of p53 and Pten after knockdown of miR-221/222 in MDA-MB-231 cells. β-actin served as loading control. (E) Schematic diagram of the mechanism through which miR-221/222 regulates cell sensitivity to cisplatin in MDA-MB-231 cells. Data are mean ± SEM (n = 3). *p < 0.05, **p < 0.01.