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. 2020 Jan 20;10:732. doi: 10.1038/s41598-019-57292-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein expression for P GHRH-R in mouse prefrontal cortex exposed to subcutaneous chronic treatment for 4 weeks (n = 3 for each group of treatment), assessed by Western blot. Actin served as internal control. Data are expressed as means ± S.E.M. and analyzed by analysis of variance (ANOVA) followed by Bonferroni post-hoc test.*p < 0.05 vs vehicle. The grouping of gels is cropped from different parts from different gels.