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. 2020 Jan 20;10:689. doi: 10.1038/s41598-019-56797-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cold priming and memory in cold response and memory mutants. (a) Schematic illustration of the growth conditions. Analysis of freezing resistance by measurement of electrolyte leakage (LT₅₀) in the cbfs (b), erf105 (c) and the fgt1 mutant (d). 21-d-old seedlings were analysed after three days cold-priming at 4 °C (P3), after a subsequent lag phase of three days at 22 °C (L3) and after an additional 4 °C cold triggering stimulus for one day (T1). Cold-primed and -triggered seedlings (P3L3T1) were compared to only cold-triggered plants (T1, dashed line). Results for in parallel tested wild-type Col-0 control plants are shown as striped columns. Significance differences of the mutant genotype between conditions were tested by one-way ANOVA (small letters), between the mutant genotype and Col-0 (C and P3) by two-way ANOVA (capital letters). Identical letters indicate no significant difference (p < 0.05). n = 4. C, control; DAS, days after sowing.