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. 2020 Jan 20;10:634. doi: 10.1038/s41598-020-57428-6

Figure 4.

Figure 4

Degradation of PLIN1 by CTSB. (A–D) Total cell lysates extracted from Mock or CTSB-OE 3T3L1 adipocytes were analysed by immunoblotting using anti-PLIN1, PLIN2, CIDEC, FLAG, and LMNB1 antibodies (A) and quantified (B–D). Representative images and quantitative data (n = 4) are shown. Intensity of LMNB1 was used as a loading control. Values indicate the mean ± SD. Differences between values were analysed by Student’s t-test with *P < 0.05, **P < 0.01. (E,F) Mock or CTSB-OE 3T3L1 adipocytes were treated with 10 μM CA074ME for 24 h. Total cell lysates were analysed by immunoblotting using anti-PLIN1, CIDEC, and LMNB1 antibodies (E) and quantified (F). Representative images and quantitative data (n = 4) are shown. Intensity of LMNB1 was used as a loading control. Values indicate the mean ± SD. Differences between values were analysed by Tukey-Kramer method with *P < 0.05, **P < 0.01. (G) Mock or CTSB-OE 3T3L1 adipocytes were treated with 10 μM CA074ME for 24 h. Immunofluorescence analysis was performed with an anti-PLIN1 antibody. PLIN1 (green), lipid droplets (red) and nucleus (blue) are shown. Data shown are representative of individual experiments. Scale bars represent 20 μm.