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. 2020 Jan 20;10:634. doi: 10.1038/s41598-020-57428-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dysregulation of lipid metabolism by CTSB overexpression in 3T3L1 adipocytes. (A and B) Lipid droplets (LDs) were stained by Nile red in Mock or CTSB-OE 3T3L1 adipocytes. Representative images (A) and quantitative data of the mean LD diameter (B) are shown. Scale bar represents 50 μm. Values indicate the mean ± SD. Differences between values were analysed by Student’s t-test with *P < 0.05, **P < 0.01. (C and D) Release assays for glycerol (C) and measurement of intracellular TG content (D) were performed in Mock or CTSB-OE 3T3L1 adipocytes. Values indicate the mean ± SD. Differences between values were analysed by Tukey-Kramer method with *P < 0.05, **P < 0.01.