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. 2020 Jan 20;10:709. doi: 10.1038/s41598-019-57388-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endogenous syntaxin-3 mRNA expression in CA1 neurons is very low and dissipates in syntaxin-3 cKO mice. (a-c) In situ hybridization using syntaxin-3 3′ half-antisense probe (a), DAPI staining (b), and merged signals (c) in control brain slice. Antisense staining in (c) was shown in green. (d,e) Higher magnification of the hippocampal region in control (d) and cKO (e) brain slices. Arrows indicate the area of CA1 neurons. Scale: 500 µm.