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. 2020 Jan 20;10:709. doi: 10.1038/s41598-019-57388-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Syntaxin-3 cKO exbibits normal long-term potentiation. (a,b) Average fEPSPs slope (a) in control and syntaxin-3 cKO slices (b) 20 min before (1), immediately after theta burst stimulation (15 bursts of 4 pulses at 100 Hz with interburst internal of 200 msec) (2), and 60 min after (3). Throughout recording, the stimulation intensity was set to give a baseline fEPSP slope 30% of maximum evoked slopes. Error bars indicate SEM (animals: n = 10 for control and 11 for syntaxin-3 cKO). N.S. indicates nonsignificant differences between the two groups (p > 0.05); two-sample t-test. (c) Representative recordings of fEPSPs of control and syntaxin-3 cKO at baseline (1), immediately after LTP induction (2) and 50 min after LTP induction (3).