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. 2020 Jan 20;10:709. doi: 10.1038/s41598-019-57388-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Syntaxin-3 cKO mice exhibit fear conditioning which is indistinguishable from control mice. Freezing time ratio (%) between control (n = 5) and syntaxin-3 cKO (n = 5) mice before shocks, aftershocks, 2nd day post shocks and 7^th day post shocks. Control and syntaxin-3 cKO groups exhibited increased freezing behavior after the administration of electrical shock. Both groups retained the increased freezing behavior one and seven days after conditioning. Statistical analyzes found no significant differences (p > 0.05) in freezing behavior of syntaxin-3 flox control mice and syntaxin-3 cKO mice. N.S. indicates nonsignificant; mixed ANOVA test.