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. 2020 Jan 20;10:651. doi: 10.1038/s41598-020-57506-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of NAMPT inhibition on intracellular NAD⁺ content in cancer cell lines with different basal levels of CD73 protein. (A) Intracellular NAD⁺ levels in cells treated with FK866 (30 nM) and/or nicotinamide (NAM) (100 μM), as compared to untreated controls. (B) Intracellular NAD⁺ levels in cells treated with FK866 (30 nM) and/or nicotinamide riboside (NR) (100 μM), as compared to untreated controls. (C) Intracellular NAD⁺ levels in cells treated with FK866 (30 nM) and/or nicotinamide mononucleotide (NMN) (100 μM), as compared to untreated controls. (D) Top panel: Representative immunoblot analysis of NRK1 expression to correlate the expression of NRK1 in four cancer cell lines (see Supplement Fig. 3E for additional immunoblot figures). Bottom panel: Densitometry analysis of 3 independent immunoblots, performed using Image Lab software. Statistical analysis was performed using GraphPad Prism 7 - one or two-way ANOVA followed by post-hoc test with Tukey’s correction was used (ns = not significant, *p = 0.0176, **p = 0.0018, ***p = 0.0009 or ****p < 0.0001).