Table 1.
–mRNA expression of genes involved in NAD+ metabolism.
| Gene Symbol | Protein symbol | LN428 | T98G | A375 | G09607 | HCT116 | MCF-7 | MDA-MB-231 | NCI.H460 | U87MG | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| De novo pathway | AFMID | AFMID | 40.89 | 45.79 | 33.45 | 66.57 | 204.48 | 55.25 | 51.44 | 73.62 | 57.21 |
| HAAO | HAAO | 29.49 | 37.08 | 25.96 | 24.75 | 25.32 | 25.01 | 26.06 | 27.74 | 26.41 | |
| IDO1 | IDO1 | 14.88 | 18.55 | 10.28 | 10.23 | 11.46 | 11.02 | 11.57 | 10.55 | 10.92 | |
| IDO2 | IDO2 | 29.80 | 33.33 | 28.09 | 26.69 | 31.94 | 26.52 | 28.48 | 31.50 | 28.45 | |
| KMO | KMO | 83.45 | 14.30 | 7.36 | 7.41 | 7.82 | 7.07 | 7.65 | 8.99 | 7.57 | |
| KYNU | KYNU | 57.84 | 2249.41 | 170.52 | 7.52 | 7.02 | 1983.97 | 483.52 | 2515.09 | 698.91 | |
| QPRT | QPRT | 52.12 | 38.65 | 29.42 | 19.19 | 17.89 | 178.43 | 18.17 | 19.16 | 64.10 | |
| TDO2 | TDO2 | 17.21 | 33.97 | 11.03 | 11.17 | 8.72 | 8.01 | 13.09 | 297.70 | 607.43 | |
| Salvage pathway | NMRK1 | NRK1 | 17.32 | 15.70 | 13.43 | 107.94 | 19.98 | 51.06 | 15.13 | 92.69 | 64.43 |
| NMRK2 | NRK2 | 17.81 | 17.06 | 14.38 | 13.60 | 14.25 | 13.87 | 15.07 | 13.26 | 13.58 | |
| NADK | NADK | 277.26 | 122.72 | 282.80 | 245.63 | 275.73 | 324.26 | 168.87 | 174.61 | 206.54 | |
| NADSYN1 | NADSYN1 | 55.78 | 89.77 | 52.15 | 83.67 | 56.34 | 227.16 | 88.74 | 87.07 | 147.14 | |
| NAMPT | NAMPT | 576.38 | 806.05 | 835.90 | 306.31 | 320.68 | 205.40 | 690.60 | 2235.86 | 1237.03 | |
| NAPRT | NAPRT1 | 30.58 | 43.24 | 34.38 | 160.61 | 254.06 | 123.02 | 118.04 | 42.58 | 33.53 | |
| NMNAT1 | NMNAT1 | 40.97 | 25.39 | 57.95 | 49.21 | 52.60 | 46.96 | 39.11 | 27.37 | 73.35 | |
| NMNAT2 | NMNAT2 | 61.85 | 66.41 | 36.01 | 63.98 | 19.56 | 38.67 | 132.46 | 78.27 | 197.11 | |
| NMNAT3 | NMNAT3 | 47.09 | 31.96 | 18.85 | 18.99 | 32.23 | 24.09 | 20.71 | 20.94 | 19.06 | |
| PNP | PNP | 851.34 | 221.99 | 394.01 | 656.57 | 648.50 | 634.86 | 1424.01 | 1122.68 | 383.69 | |
| NAD+ consuming enzymes | BST1 | CD157 | 19.21 | 8.86 | 8.31 | 138.09 | 9.43 | 7.45 | 11.74 | 111.67 | 25.21 |
| CD38 | CD38 | 10.62 | 9.63 | 9.04 | 622.73 | 8.14 | 8.72 | 8.74 | 8.36 | 8.41 | |
| ENTPD1 | CD39 | 17.57 | 18.20 | 17.15 | 16.72 | 19.28 | 16.32 | 21.65 | 18.77 | 16.39 | |
| NNMT | NNMT | 448.21 | 731.95 | 1127.63 | 565.88 | 24.03 | 18.71 | 26.03 | 154.81 | 410.01 | |
| NT5E | CD73 | 1504.20 | 19.21 | 689.83 | 1438.59 | 469.60 | 90.63 | 2247.53 | 379.95 | 1033.97 | |
| PARP1 | PARP1 | 518.96 | 604.91 | 398.42 | 1033.13 | 639.85 | 415.14 | 680.80 | 794.18 | 129.65 | |
| PARP2 | PARP2 | 267.44 | 206.39 | 206.23 | 299.33 | 412.53 | 266.18 | 385.40 | 353.05 | 119.51 | |
| PARP3 | PARP3 | 196.23 | 177.90 | 163.03 | 238.20 | 127.02 | 38.35 | 141.02 | 116.70 | 239.57 | |
| SIRT1 | SIRT1 | 395.87 | 311.12 | 182.53 | 360.42 | 411.73 | 326.82 | 342.83 | 341.26 | 313.82 | |
| SIRT2 | SIRT2 | 332.45 | 228.72 | 230.47 | 255.80 | 250.11 | 513.48 | 338.45 | 323.30 | 299.61 | |
| SIRT3 | SIRT3 | 208.19 | 278.25 | 94.12 | 98.92 | 169.51 | 87.97 | 120.82 | 149.62 | 129.08 | |
| SIRT4 | SIRT4 | 18.43 | 21.91 | 14.83 | 14.85 | 17.95 | 13.93 | 14.91 | 14.86 | 13.02 | |
| SIRT5 | SIRT5 | 62.25 | 47.84 | 16.97 | 36.65 | 58.62 | 47.85 | 52.25 | 31.19 | 30.79 | |
| SIRT6 | SIRT6 | 63.69 | 67.00 | 64.35 | 57.34 | 65.25 | 75.76 | 60.34 | 60.45 | 70.75 | |
| SIRT7 | SIRT7 | 268.57 | 249.30 | 116.31 | 225.22 | 271.26 | 263.94 | 188.41 | 180.28 | 236.76 | |
| TNKS | TNKS | 71.42 | 196.13 | 155.65 | 92.31 | 165.27 | 87.94 | 77.52 | 78.46 | 220.74 | |
| TNKS2 | TNKS2 | 28.17 | 128.73 | 96.43 | 137.27 | 175.87 | 84.43 | 109.39 | 103.54 | 121.72 |