Figure 2.

Increased levels of p-S6K1 are associated with radio-resistant CD44^high/CD24^low MCF7 cells. (a) Cells were analysed with immunoblotting and the indicated antibodies. (b) and (c) Control MCF7 cells and CD44^high/CD24^low MCF7 cells were pre-treated without or 10 nM everolimus and then treated with 0, 3, or 6 Gy irradiation for 42 h. Cells were analysed with immunoblotting and the indicated antibodies (b). The number of colonies was measured (c). Data represent typical results and are presented as the mean ± standard deviation of three independent experiments. Arrows indicate the signal from S6K1 and p-S6K1. Blotting results in (a) and (b) were cropped from different gels and are therefore delineated by white spaces and lines. The original blot data are shown in Supplementary Figures S3. N.S.: not significant; H/E: high exposure; L/E: low exposure. *p < 0.01 based on a paired t-test.