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. 2020 Jan 20;10:671. doi: 10.1038/s41598-020-57510-z

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative abundance (RA) of various HR O-glycoforms in IgA1 isolated from sera of 10 healthy controls. (a) RA determined by using an automated program, Glycan Analyzer. RA of each O-glycopeptide is shown as a box plot with glycoforms on X axis in decreasing order of abundance. The number of attached GalNAc (x) and Gal (y) residues is notes as x_y on X axis. The number of Gd O-glycans in each O-glycopeptide is shown above the box. The upper and lower limits of the boxes represent the first and third quartiles of RA%. A segment inside the rectangle shows the median and the whiskers above and below the box show the minimum and maximum values. (b) Correlation between RA determined by Glycan Analyzer and by manual assessment. Linear regression analysis revealed a strong congruence between the two methods (y = 0.994x + 0.022, R² = 0.9931).