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. 2020 Jan 20;10:666. doi: 10.1038/s41598-019-57299-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of MPO-H₂O₂-Cl⁻ system on integrity of native HCASMC-ECM proteins. Decellularized native ECM was exposed to MPO-H₂O₂-Cl⁻ system (20 nM MPO, 0–100 μM H₂O_2, 200 mM Cl⁻) at 37 °C for 2 h. The presence of HOCl-induced modifications to matrix proteins were detected via double immunofluorescence staining using fibronectin pAb (green) and mAb 2D10G9 (red), respectively. An Alexa Fluor 488-conjugated anti-rabbit secondary antibody (green, top row) and an Alexa Fluor 594-conjugated anti-mouse secondary antibody (red, middle row) were used to visualize matrix protein-primary antibody complexes. Merged images are shown in the bottom row. Representative images from n = 3 independent experiments are shown. Scale bars: 50 μm.