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. 2020 Jan 20;10:678. doi: 10.1038/s41598-020-57529-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of carP deletion in F. oxysporum. (A) Aspect of surface colonies of the wild strain and a representative ΔcarP mutant grown on minimal medium for 1 week under light. (B) Carotenoid content in the wild strain and mutants ΔcarP* and ΔcarP grown for 1 week in the dark or under light. (C–F) Transcript levels for the carRA, carB, carO, carX, carD, carT, carS, and carP genes in the same strains grown in the dark or exposed for 1 hour to light. RT-qPCR data show the mean and standard error of three independent experiments. Relative mRNA levels refer to the mRNA content of the wild strain in the dark. Differences found to be significant according to the t tests are indicated (P-values, *p < 0.033; **p < 0.002; ***p < 0.001).