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. 2020 Jan 20;10:643. doi: 10.1038/s41598-019-57218-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmap for the 395 immune-related genes from 21 patients. (a) Columns represent patients and rows genes. Expression levels have been aligned according to functional annotation groups. Categories of response (DCB, NDB), PD-L1 expression (≥50%,1–50%, <1%), smoking status (smoker, non-smoker) and histologic subtypes (adenocarcinoma, squamous cell carcinoma, large cell neuroendocrine carcinoma, adenosquamous cell carcinoma) are shown, and tumor infiltrating lymphocytes (TILs) and tumor mutation burden (TMB) are shown in continuous variables. (b) Heatmap for five signature scores of functional annotation groups discriminating DCB and NDB patients. (c) Heatmap for two gene signature scores and two genes that discriminate DCB and NDB patients.