Skip to main content
. 2019 Dec 24;23(1):100794. doi: 10.1016/j.isci.2019.100794

Figure 5.

Figure 5

Molecular Mechanisms Involved in the Effects of Chronic THC and CBD: DARPP-32

Monkeys (N = 3 per group) were treated chronically with vehicle, THC, or THC + CBD.

(A and B) Western blot analysis of homogenates from NAc (A) or CPu (B) using an antibody raised against phospho-threonine-75-DARPP-32 (pT75-DARPP-32) was conducted. Representative images (top) and quantification of the density (bottom) are shown. Data are mean ± SD.

(C and D) Analysis of pT75-DARPP-32 using PLA from monkeys treated as in (A). PLA was performed using anti-DARPP-32 and anti-pT75-DARPP-32, with the PLA recognizing DARPP-32 only when pT75 was activated. Representative images from each group of monkeys are shown in (C). (D) Graph summarizing the quantification of PLA-pT75-DARPP-32 using Duolink software. Number of neurons analyzed from each group is indicated. Data are mean ± SEM. One-way ANOVA followed by Bonferroni correction was used to compare statistical differences. (**p < 0.01; ***p < 0.001). Scale bars, 10 μm.