Figure 6.

Molecular Mechanisms Involved in the Effects of Chronic THC and CBD: ERK1/2 and GSK-3

(A–D) Monkeys (N = 3 per group) were treated chronically with vehicle, THC, or THC + CBD. Analysis by western blot of homogenates from the NAc (left panels) or CPu (right panels) using antibodies to phospho-ERK (pERK, A and B) or phospho-GSK3 (pGSK, C and D). GAPDH was used as a loading control. Representative images (top) and quantification of the density (bottom) are shown. Data are mean ± SD. Two-way ANOVA followed by Bonferroni correction was used to compare statistical differences. (*p < 0.05; **p < 0.01; ***p < 0.001).